CJD onderzoek in het Instituut Born-Bunge
Creutzfeldt-Jakob disease (CJD) is a neurodegenerative disease that belongs to the category of the transmissible spongiform encephalopathies. CJD is transmissible within a species but also in between species for example from humans to apes. Our laboratory has extensive experience with the classic neuropathological diagnosis of CJD. New probes presently allow the development of antibody based laboratory tests. In Belgium there are no clinical laboratories where this type of laboratory test is routinely performed. The Health Council has recommended the development of a methodology to enable a differential diagnose of CJD.
We developed immunohistochemical protocols to detect at least one of these markers in brain with a very high degree of reliability.
The materials necessary to develop these tests are available in the brain-bank and CSF sample collection of our laboratory or by collaboration with other laboratories. These collections contain samples of CJD patients as well as samples of patients that suffer from other neurodegenerative diseases, for example Alzheimer's disease and healthy controls. Our laboratory also possesses antibodies against the CJD markers and the technical know-how about immunoassays and immunohistochemical techniques.
Technische richtlijnen en formulieren
Resultaten van de 14-3-3 bepaling
Sensitiviteit en specificiteit
Overzicht stalen
Diagnostische criteria voor sporadische CJD (Rotterdam, 1998)
Contactgegevens
Links
Resultaten van de 14-3-3 bepaling
Sensitiviteit en specificiteit: detectie Biochemische merkers
Gebruik makend van alle 14-3-3 banden
Sensitiviteit: 98%
Specificiteit
t.o.v. andere dementies: 95%
t.o.v. alle categoriën: 91%
Indien zwak positief als negatief wordt beschouwd:
Sensitiviteit: 89%
Specificiteit
t.o.v. andere dementies: 99%
t.o.v. alle categoriën: 95%
Overzicht stalen
| 14-3-3 Analysis | |||
|---|---|---|---|
| Positive | Negative | Total | |
| CJD definite | 94 | 2 | 96 |
| CJD probable | 42 | 1 | 43 |
| CJD possible | 12 | 15 | 27 |
| CJD familial | 4 | 2 | 6 |
| TOTAL CJD | 140 | 5 | 145 |
| Alzheimer’s disease | 6 | 120 | 126 |
| Dementia (not AD) | 8 | 141 | 149 |
| TOTAL Dementia | 14 | 261 | 275 |
| Other neurological disorders | 13 | 126 | 139 |
| No final diagnosis | 10 | 101 | 111 |
| Paraneoplastic disorders | 4 | 15 | 19 |
| Psychicatric disorders | 1 | 16 | 17 |
| Vascular disorders | 1 | 12 | 13 |
| Viral encefalitis | 14 | 14 | 28 |
| TOTAL Other disorders | 43 | 284 | 327 |
| TOTAL | 209 | 565 | 774 |
| Percentile results | |||
| Positive | Negative | Total | |
| CJD definite | 98% | 2% | 100% |
| CJD probable | 98% | 2% | 100% |
| CJD possible | 44% | 56% | 100% |
| CJD familial | 67% | 33% | 100% |
| TOTAL CJD | 97% | 3% | 100% |
| Alzheimer’s disease | 5% | 95% | 100% |
| Dementia (not AD) | 5% | 95% | 100% |
| TOTAL Dementia | 5% | 95% | 100% |
| Other neurological disorders | 9% | 91% | 100% |
| No final diagnosis | 9% | 91% | 100% |
| Paraneoplastic disorders | 21% | 79% | 100% |
| Psychicatric disorders | 6% | 94% | 100% |
| Vascular disorders | 8% | 92% | 100% |
| Viral encefalitis | 50% | 50% | 100% |
| TOTAL Other disorders | 13% | 87% | 100% |
| TOTAL | 27% | 73% | 100% |
Diagnostische criteria voor sporadische CJD (Rotterdam, 1998)
1. Diagnostische symptomen
I - snel progressieve dementie
II - klinische symptomen
A - myoclonus
B - visuele of cerebellaire problemen
C - pyramidale of extrapyramidale tekens
D - akinetisch mutisme
III - onderzoeken
A - typisch EEG
B - positieve 14-3-3
2. Diagnostische status
Probable CJD: I en 2 van II en III A en/of B
Possible CJD: I en 2 van II en dit gedurende een periode van minder dan 2 jaar
Definite CJD: neuropathologisch/immunohistochemisch bevestigd
Contactgegevens
| Prof. Dr. Patrick Cras | Ing. Bart De Vil |
|---|---|
| mail prof. Cras | mail Bart De Vil |
| Tel: +32 3 821 57 57 | Tel: +32 3 265 25 97 or +32 3 265 26 05 Fax: +32 3 265 26 69 |
| Laboratorium voor Neurobiologie Universiteit of Antwerpen CDE Universiteitsplein 1, Gebouw T, Lokaal 5.20 BE-2610 Antwerpen, België |
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